Tunable Wavelength (Tune) Detection Cartridge - Molecular Devices SpectraMax Paradigm User Manual

Tunable Wavelength (TUNE) Detection Cartridge

The Tunable Wavelength (TUNE) Detection Cartridge enables several detection modes,
including:
Fluorescence Intensity (FL), see
Time-Resolved Fluorescence (TRF), see
Luminescence (LUM), see
The spectral optimization feature of the Tunable Wavelength (TUNE) Detection Cartridge can
help to get the maximum signal-to-background ratio for most fluorophores or luminescence
labels that are compatible with the wavelength ranges.
Using a Spectrum read, you can define a fixed excitation wavelength and scan the emission
wavelengths, or define a fixed emission wavelength and scan the excitation wavelengths. The
spectral optimization helps to get the maximum signal-to-background ratio from a
fluorescent or fluorescently labeled analyte. For more information on using the spectral
optimization feature, see the SoftMax Pro Software application help or user guide.
The Tunable Wavelength (TUNE) Detection Cartridge occupies three (3) slots in the detection
cartridge drawer. It can be installed in either the top detection cartridge drawer for top
reading, or in the bottom detection cartridge drawer for bottom reading.
For installation instructions, see
Note:Molecular Devices recommends that you install the Tunable Wavelength (TUNE)
Detection Cartridge in the top detection cartridge drawer when running time-resolved
fluorescence or glow luminescence read modes.
The can do the following read types:
End Point, see
Kinetic, see
Well Scan, see
Spectrum, see
You can create protocols that use the Tunable Wavelength (TUNE) Detection Cartridge in the
SoftMax Pro Software. For information on creating protocols, see "Creating a Protocol" in
the SoftMax Pro Software application help or user guide.
You can use the Protocol Manager in the SoftMax Pro Software to quickly find and open a
predefined protocol.
More protocols and updated protocols can be downloaded from the Knowledge Base on the
Molecular Devices support web site (www.moleculardevices.com/support) or from the
protocol sharing web site (www.softmaxpro.org).
5014038 E
About Fluorescence Intensity on page
About Luminescence on page
Installing a Detection Cartridge on page
Endpoint Read Type on page
Kinetic Read Type on page
Well Scan Read Type on page
Spectrum Read Type on page
Chapter 5: Available Detection Cartridges
About Time-Resolved Fluorescence on page
99.
20.
20.
21.
21.
98.
99.
84.
97