Dual Color Luminescence (Lum) (Chroma-Glo) Detection Cartridge - Molecular Devices SpectraMax Paradigm User Manual

SpectraMax Paradigm Multi-Mode Detection Platform User Guide

Dual Color Luminescence (LUM) (Chroma-Glo) Detection Cartridge

The Dual Color Luminescence (LUM) (Chroma-Glo) Detection Cartridge enables read modes
for luminescence and is designed to provide the optimal performance when used with
Promega Chroma-Glo™ reagents.
For luminescence measurements that do not require emission filters, see
Luminescence (LUM) Detection Cartridges on page
The Dual Color Luminescence (LUM) (Chroma-Glo) Detection Cartridge occupies one (1) slot
in the detection cartridge drawer. It can be installed in either the top detection cartridge
drawer for top reading, or in the bottom detection cartridge drawer for bottom reading.
For installation instructions, see
The can do the following read types:
End Point, see
Kinetic, see
Well Scan, see
You can create protocols that use the Dual Color Luminescence (LUM) (Chroma-Glo)
Detection Cartridge in the SoftMax Pro Software. For information on creating protocols, see
"Creating a Protocol" in the SoftMax Pro Software application help or user guide.
You can use the Protocol Manager in the SoftMax Pro Software to quickly find and open a
predefined protocol.
More protocols and updated protocols can be downloaded from the Knowledge Base on the
Molecular Devices support web site (www.moleculardevices.com/support) or from the
protocol sharing web site (www.softmaxpro.org).
Chroma-Glo is a trademark of Promega Corporation.
About Dual Color Luminescence
Luminescence is the emission of light by processes that derive energy from essentially non-
thermal changes, the motion of subatomic particles, or the excitation of an atomic system by
radiation. Luminescence detection relies on the production of light from a chemical reaction
in a sample.
In a dual color luminescence read mode, dual labels (such as gene reporters) causing the
emission of different wavelength bands at the same time are measured.
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Note: This detection cartridge cannot be installed in the rear-most slot in either the
top or bottom detection cartridge drawer.
Note:Molecular Devices recommends that you install the Dual Color Luminescence
(LUM) (Chroma-Glo) Detection Cartridge in the top detection cartridge drawer when
running luminescence (LUM) read modes.
Endpoint Read Type on page
Kinetic Read Type on page
Well Scan Read Type on page
128.
Installing a Detection Cartridge on page
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Glow
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