Molecular Devices SpectraMax Paradigm User Manual page 103

Chapter 5: Available Detection Cartridges
About Fluorescence Intensity
In fluorescence intensity read mode, the source light is directed through a tunable excitation
filter and then focused by an objective lens from above or below the microplate. The filter
passes only the specified wavelength band necessary to excite samples. The objective lens
collects the resulting fluorescence and directs it through an emission filter to separate
background light from the specific wavelengths generated by samples. This signal is detected
by a photo multiplier tube.
The Multi-Mode (MULTI) Detection Cartridge contains filter sets for measuring fluorescence
intensity of coumarin, fluorescein, rhodamine, and Texas Red. The Multi-Mode (MULTI)
Detection Cartridge uses high-power LEDs and optimized filter sets together with the photon
counting detection of the instrument, resulting in excellent detection limits and linear
dynamic range at short read times. See Measurement Specifications on page 105.
For more information about fluorescence intensity, see Fluorescence Intensity Read Mode
on page 28.
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