Molecular Devices SpectraMax Paradigm User Manual page 54

SpectraMax Paradigm Multi-Mode Detection Platform User Guide
Applications of AlphaScreen
AlphaScreen reagent and assays are used for drug discovery purposes. Examples of
AlphaScreen assays include:
G-protein coupled receptor (GPCR) assay kits, for cAMP quantification or IP3
quantification.
Tyrosine Kinase assays.
Cytokine detection kits, such as TNF-alpha detection (immunoassay).
AlphaScreen read mode can also capture the Europium emission line of AlphaLISA®.
You can use the Protocol Manager in the SoftMax Pro Software to quickly find and open a
predefined protocol.
More protocols and updated protocols can be downloaded from the Knowledge Base on the
Molecular Devices support web site (www.moleculardevices.com/support) or from the
protocol sharing web site (www.softmaxpro.org).
To do AlphaScreen reads, the instrument requires
page 107.
For more information, go to www.perkinelmer.com.
ALPHASCREEN and ALPHALISA are registered trademarks of PerkinElmer, Inc.
Analyzing AlphaScreen Data
The conversion rate of photons to counts and relative fluorescence units (RFU) is individual
for each reader. Therefore, raw data from the same microplate can seem to be different from
one instrument to the next. Also, the data format used by instrument manufacturers might
be counts normalized per second or not normalized counts, and therefore the raw data can
be different by several orders of magnitude. It is important to know that the number of
counts and the size of figures is in no way an indication of sensitivity. See
page 55.
The raw data can be normalized to counts per second by selecting the Normalization option
in the SoftMax Pro Software Settings dialog. See "Creating a Protocol" in the SoftMax Pro
Software application help or user guide.
Analyzing and validating AlphaScreen data can consist of the following:
Background Correction on page 55
Detection Limit on page 55
Data Qualification and Validation on page 56
54
AlphaScreen Detection Cartridges , see
Detection Limit on
5014038 E