Molecular Devices SpectraMax Paradigm User Manual page 105

Microplate Recommendations
The following information is specific to this detection cartridge. For general microplate
selection and handling guidelines, see
Table 5-5: Microplate Selection Guidelines for the Multi-Mode (MULTI) Detection
Cartridge
Read Mode
Fluorescence Intensity
(FL) top read
Fluorescence Intensity
(FL) bottom read
Time-Resolved
Fluorescence (TRF)
Luminescence (LUM)
Note: White microplates provide significantly higher signal than black microplates,
and are recommended if high sensitivity is required. White microplates can, however,
exhibit some detectable phosphorescence which increases background after being
exposed to light (in particular under neon lights). For maximum sensitivity, Molecular
Devices recommends to prepare microplates under reduced ambient light conditions,
and to adapt the microplates to dark for 10 to 30 minutes before measurement.
Measurement Specifications
The specifications for measurements using the Multi-Mode (MULTI) Detection Cartridge are
shown in the following table.
Table 5-6: Measurement Specifications for the Multi-Mode (MULTI) Detection Cartridge
Item
Detection cartridge name
Short name
Part number
Weight
Read Modes
5014038 E
Selecting Suitable Microplate Types on page
Microplate Other Considerations
Type
Solid black When an application specifies a surface treatment, use
only microplates with the correct treatment.
Solid black, When an application specifies a surface treatment, use
clear bottom only microplates with the correct treatment.
Solid white When an application specifies a surface treatment, use
only microplates with the correct treatment.
Solid white When an application specifies a surface treatment, use
only microplates with the correct treatment.
Molecular Devices recommends using an unlidded plate
for luminescence measurements.
Description
Multi-Mode (MULTI) Detection Cartridge
MULTI
0200-7001
3.1 lbs (1.4 kg)
Fluorescence Intensity (FL)
Time-Resolved Fluorescence (TRF)
Luminescence (LUM)
Chapter 5: Available Detection Cartridges
91.
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