Molecular Devices SpectraMax Paradigm User Manual page 20

SpectraMax Paradigm Multi-Mode Detection Platform User Guide
Endpoint Read Type
In an Endpoint read, a reading of each microplate well is taken in the center of each well, at a
single wavelength or at multiple wavelengths. Depending on the read mode, raw data values
are reported as optical density (OD), %Transmittance (%T), relative fluorescence units (RFU),
or relative luminescence units (RLU).
Kinetic Read Type
In a Kinetic read, the instrument collects data over time with multiple readings taken in the
center of each well at regular intervals.
The values calculated based on raw kinetic data include VMax, VMax per Sec, Time to VMax,
and Onset Time. Kinetic readings can be single-wavelength or multiple-wavelength readings.
Kinetic analysis can be done for up to 99 hours. The kinetic read interval depends on the
instrument setup parameters selected in the SoftMax Pro Software.
Kinetic analysis has many advantages when determining the relative activity of an enzyme in
different types of microplate assays, including ELISAs and the purification and
characterization of enzymes and enzyme conjugates. Kinetic analysis is capable of providing
improved dynamic range, precision, and sensitivity relative to endpoint analysis.
Peak Pro™ Analysis functions provide advanced peak detection and characterization for
applicable kinetic reads. See the SoftMax Pro Software Formula Reference Guide.
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