Chapter 2: Read Modes And Read Types; Supported Read Types - Molecular Devices SpectraMax Paradigm User Manual

Chapter 2: Read Modes and Read Types

The detection capabilities of the SpectraMax Paradigm Multi-Mode Detection Platform are
determined by the installed detection cartridges. Up to six detection cartridges can be
installed in each of the two detection cartridge drawers. For information about detection
cartridges, see
The software detects the installed cartridge configuration and does all measurement types
supported by the detection cartridges. Use the SoftMax Pro Software to define the
parameters for the read mode and read type of your assay. See the SoftMax Pro Software
application help or user guide.
Application notes with specific application protocol suggestions can be found in the
Information Center and the Knowledge Base on the Molecular Devices web site at
www.moleculardevices.com.
For more information on the supported read modes, see the following topics:
Absorbance Read Mode on page 23
Fluorescence Intensity Read Mode on page 28
Luminescence Read Mode on page 33
Time-Resolved Fluorescence Read Mode on page 38
HTRF Read Mode on page 45
FRET Read Mode on page 44
Fluorescence Polarization Read Mode on page 49
AlphaScreen Read Mode on page 53
Western Blot TRF Read Mode on page 57

Supported Read Types

For most read modes, endpoint, kinetic, multi-point well-scan, and spectrum microplate
applications can be set up and run with the SoftMax Pro Software.
For more information on the supported read types, see the following topics:
Endpoint Read Type on page 20
Kinetic Read Type on page 20
Well Scan Read Type on page 21
Spectrum Read Type on page 21
Membrane Read Type on page 22
5014038 E
Available Detection Cartridges on page 93.
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