Cisbio Htrf Detection Cartridge - Molecular Devices SpectraMax Paradigm User Manual

SpectraMax Paradigm Multi-Mode Detection Platform User Guide

Cisbio HTRF Detection Cartridge

The Cisbio HTRF Detection Cartridge uses a high-energy Xenon flash lamp for sensitive
reading of HTRF reagents. Also, the standard dual-emission design gives the most accurate
results in short overall read time. This detection cartridge enables time-resolved fluorescence
read modes for europium cryptate and europium acceptors.
The fluorescence ratio related to the HTRF readout is a correction method developed by
Cisbio, for which Cisbio has granted a license to Molecular Devices. Its application is strictly
limited to the use of HTRF reagents and technology, excluding other TR-FRET technologies
such as IMAP TR-FRET calculations of acceptor to donor ratios.
The Cisbio HTRF Detection Cartridge is a dual-emission detection cartridge and occupies two
(2) slots in the detection cartridge drawer. It can be installed only in the top detection
cartridge drawer for top reading.
For installation instructions, see
The Cisbio HTRF Detection Cartridges can do the following read types:
End Point, see
Kinetic, see
Well Scan, see
You can create protocols that use the Cisbio HTRF Detection Cartridges in the SoftMax Pro
Software. For information on creating protocols, see "Creating a Protocol" in the SoftMax
Pro Software application help or user guide.
You can use the Protocol Manager in the SoftMax Pro Software to quickly find and open a
predefined protocol.
More protocols and updated protocols can be downloaded from the Knowledge Base on the
Molecular Devices support web site (www.moleculardevices.com/support) or from the
protocol sharing web site (www.softmaxpro.org).
HTRF is a registered trademark of Cisbio Bioassays.
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Installing a Detection Cartridge on page
Endpoint Read Type on page
Kinetic Read Type on page
Well Scan Read Type on page
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