Molecular Devices SpectraMax Paradigm User Manual page 106

SpectraMax Paradigm Multi-Mode Detection Platform User Guide
Table 5-6: Measurement Specifications for the Multi-Mode (MULTI) Detection Cartridge
(continued)
Item
Type
Number of slots
Light source
Filter set
Labels
Detection limit, Top
optimized
Detection limit, Top
guaranteed
Linear dynamic range, Top
106
Description
Single emission
3 slots, top or bottom
High-power LEDs
TRF: pulse period = 10 X pulse length
Coumarin:
EX: 360-35; EM: 465-35
Fluorescein:
EX: 485-20; EM: 535-25
Rhodamine:
EX: 535-35; EM: 595-35
Texas Red:
EX: 585-10; EM: 635-35
Europium chelate (TRF):
EX: 370-80; EM: 616-10
Fluorescence Intensity:
Coumarin, Fluorescein, Rhodamine, Texas Red
Time-Resolved Fluorescence:
Europium Chelate
Fluorescence Intensity (Fluorescein):
96-well (200 µL): 150 amol/well (0.75 pM)
384-well (75 µL): 100 amol/well (1.3 pM)
Time-Resolved Fluorescence (Europium):
96-well (200 µL): 20 amol/well (0.1 pM)
384-well (100 µL): 10 amol/well (0.1 pM)
Luminescence (ATP):
96-well (200 µL): 0.6 fmol/well (3 pM)
384-well (50 µL): 0.3 fmol/well (6 pM)
Fluorescence Intensity (Fluorescein):
96-well (200 µL): 400 amol/well (2 pM)
384-well (75 µL): 300 amol/well (4 pM)
Time-Resolved Fluorescence (Europium):
96-well (200 µL): 25 amol/well (0.125 pM)
384-well (100 µL): 15 amol/well (0.15 pM)
Luminescence (ATP):
96-well (200 µL): 2 fmol/well (10 pM)
384-well (50 µL): 2 fmol/well (40 pM)
Fluorescein: 5 logs in a single microplate read
5014038 E