Molecular Devices SpectraMax Paradigm User Manual page 114

SpectraMax Paradigm Multi-Mode Detection Platform User Guide
About Time-Resolved Fluorescence
Time-resolved fluorescence (TRF) is a measurement technique that depends on three
characteristics that lead to better discrimination between the specific signal, proportional to
the quantity of label, and the unspecific fluorescence resulting from background and
compound interference:
Pulsed excitation light sources
Time-gated electronics faster than the fluorescence lifetime
Labels with prolonged fluorescence lifetime
The time-gating electronics introduce a delay between the cut off of each light pulse and the
start of signal collection. During the delay, the unspecific fluorescence (caused by test
compounds, assay reagents, and the microplate) vanishes while only a small portion of the
specific fluorescence from the label is sacrificed. Enough of the specific signal remains during
the decay period with the added benefit of reduced background.
The Time Resolved Fluorescence (TRF) Detection Cartridge uses an ultraviolet LED that emits
in the range between 350 nm and 380 nm for excitation of Europium chelates, and comes
equipped with emission filters for europium and samarium. This enables single-label
europium and samarium assays as well as dual-label assays involving europium and
samarium to be run. See
For more information about time-resolved fluorescence, see
Read Mode on page
Microplate Recommendations
The following information is specific to this detection cartridge. For general microplate
selection and handling guidelines, see
Table 5-12: Microplate Selection Guidelines for the Time Resolved Fluorescence (TRF)
Detection Cartridge
Read Mode
Time-Resolved
Fluorescence (TRF)
114
Measurement Specifications on page
Note: Europium cryptate and terbium require excitation wavelengths below 330 nm
and cannot be measured with this detection cartridge.
38.
Microplate
Type
Solid white
Selecting Suitable Microplate Types on page
Other Considerations
When an application specifies a surface treatment, use only
microplates with the correct treatment.
115.
Time-Resolved Fluorescence
91.
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