Molecular Devices SpectraMax Paradigm User Manual page 117

About Fluorescence Intensity
In fluorescence intensity read mode, the source light is directed through an excitation filter
and then focused by an objective lens from above or below the microplate. The filter passes
only the specified wavelength band necessary to excite samples. The objective lens collects
the resulting fluorescence and directs it through an emission filter to separate background
light from the specific wavelengths generated by samples. This signal is detected by a photo
multiplier tube.
The Fluorescence Intensity (FI) Detection Cartridges use an ultra high power LED and an
optimized filter set together with the photon counting detection capabilities of the
instrument, resulting in superior detection limits and linear dynamic range at short read
times. See
For more information about fluorescence intensity, see
on page
Microplate Recommendations
The following information is specific to this detection cartridge. For general microplate
selection and handling guidelines, see
Table 5-14: Microplate Selection Guidelines for the Fluorescence Intensity (FI) Detection
Cartridges
Read Mode
Fluorescence Intensity
(FL), top read
Fluorescence Intensity
(FL), bottom read
Measurement Specifications
The specifications for measurements using the Fluorescence Intensity (FI) Detection
Cartridges are shown in the following table.
Table 5-15: Measurement Specifications for Fluorescence Intensity (FI) Detection
Cartridges
Item
Detection
cartridge
name
Short
name
Part
5014038 E
Measurement Specifications on page
28.
Microplate
Type
Solid black
Solid black,
clear bottom
Description
Fluorescence
Intensity (FI) (coum-
fluor) Detection
Cartridge
FI-COFL
0200-7002
Chapter 5: Available Detection Cartridges
117.
Fluorescence Intensity Read Mode
Selecting Suitable Microplate Types on page
Other Considerations
When an application specifies a surface treatment, use
only microplates with the correct treatment.
When an application specifies a surface treatment, use
only microplates with the correct treatment.
Description Description
Fluorescence Fluorescence
Intensity (FI) (fluor- Intensity (FI) (Cy3-
rhod) Detection Cy5) Detection
Cartridge Cartridge
FI-FLRH FI-CY3CY5
0200-7003 0200-7004
91.
Description
Fluorescence
Intensity (FI) (CFP-
YFP) Detection
Cartridge
FI-CFP-YFP
0200-7005
117