Fluorescence Intensity (Fi) Detection Cartridges - Molecular Devices SpectraMax Paradigm User Manual

SpectraMax Paradigm Multi-Mode Detection Platform User Guide

Fluorescence Intensity (FI) Detection Cartridges

The Fluorescence Intensity (FI) Detection Cartridges use an ultra high power LED for the
excitation of various fluorescent labels, enabling fluorescence intensity read modes. Also, the
standard dual-emission design enables simple, straightforward fluorescence resonance
energy transfer (FRET) measurement.
The following Fluorescence Intensity (FI) Detection Cartridges are available:
Fluorescence Intensity (FI) (coum-fluor) Detection Cartridge
Fluorescence Intensity (FI) (fluor-rhod) Detection Cartridge
Fluorescence Intensity (FI) (Cy3-Cy5) Detection Cartridge
Fluorescence Intensity (FI) (CFP-YFP) Detection Cartridge
Other Fluorescence Intensity (FI) Detection Cartridges include the
GeneBLAzer Detection Cartridge on page 119
DL) (MultiTox-Fluor) Detection Cartridge on page
Each Fluorescence Intensity (FI) Detection Cartridge occupies one (1) slot in the detection
cartridge drawer. It can be installed in either the top detection cartridge drawer for top
reading, or in the bottom detection cartridge drawer for bottom reading.
For installation instructions, see
The Fluorescence Intensity (FI) Detection Cartridges can do the following read types:
End Point, see
Kinetic, see
Well Scan, see
You can create protocols that use the Fluorescence Intensity (FI) Detection Cartridges in the
SoftMax Pro Software. For information on creating protocols, see "Creating a Protocol" in
the SoftMax Pro Software application help or user guide.
You can use the Protocol Manager in the SoftMax Pro Software to quickly find and open a
predefined protocol.
More protocols and updated protocols can be downloaded from the Knowledge Base on the
Molecular Devices support web site (www.moleculardevices.com/support) or from the
protocol sharing web site (www.softmaxpro.org).
116
Installing a Detection Cartridge on page
Endpoint Read Type on page
Kinetic Read Type on page
Well Scan Read Type on page
and the Fluorescence Intensity Dual Label (FI-
122.
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Fluorescence Intensity (FI)
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