Molecular Devices SpectraMax Paradigm User Manual page 34

SpectraMax Paradigm Multi-Mode Detection Platform User Guide
Applications of Luminescence
Chemiluminescent or bioluminescent reactions can be induced to measure the quantity of a
particular compound in a sample. Examples of luminescent assays include the following:
Reporter gene assays (the measurement of luciferase gene expression)
Quantitation of adenosine triphosphate (ATP) as an indication of cell counts with cell-
proliferation, cytotoxicity, and biomass assays
Enzyme measurements with luminescent substrates, such as immunoassays
You can use the Protocol Manager in the SoftMax Pro Software to quickly find and open a
predefined protocol.
More protocols and updated protocols can be downloaded from the Knowledge Base on the
Molecular Devices support web site (www.moleculardevices.com/support) or from the
protocol sharing web site (www.softmaxpro.org).
The following detection cartridges have luminescence read mode capability:
Tunable Wavelength (TUNE) Detection Cartridge, see page 97
Multi-Mode (MULTI) Detection Cartridge, see page 102
Glow Luminescence (LUM) Detection Cartridges, see page 128
Dual Color Luminescence (LUM) (BRET2) Detection Cartridge, see page 131
Dual Color Luminescence (LUM) (Chroma-Glo) Detection Cartridge, see page 136
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