Molecular Devices SpectraMax Paradigm User Manual page 41

Table 2-1: Time-resolved fluorescence timing parameters example
Parameter
Pulse length
Measurement delay
Integration time per
cycle (pulse)
Integration time per
cycle (pulse)
Figure 2-4: Timing parameters for time-resolved fluorescence
5014038 E
Value
0.100 ms
0.010 ms
0.890 ms
1.890 ms
t
t
2
t
1
t
3
Comment
The period for excitation of the sample, shown as t
following figure.
This is the suggested value for the TUNE, MULTI, and TRF
detection cartridges.
The delay to ensure the excitation pulse is no longer
detectable, shown as t
2
This is the suggested value for the TUNE, MULTI and TRF
detection cartridges.
The period for accumulating the signal, shown as t
following figure.
This is the suggested value for the TUNE and MULTI detection
cartridges.
The period for accumulating the signal, shown as t
following figure.
This is the suggested value for the TRF detection cartridge.
4
Chapter 2: Read Modes and Read Types
in the following figure.
in the
1
in the
3
in the
3
41