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Summary of Contents for Molecular Devices SpectraMax MiniMax
- Page 1 SpectraMax® MiniMax™ Imaging Cytometer Installation Guide 5024111 C March 2015...
- Page 2 For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. These trademarks may not be used in any type of promotion or advertising without the prior written permission of Molecular Devices, LLC.
- Page 3 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer The SpectraMax® MiniMax™ Imaging Cytometer attaches to the bottom of the SpectraMax® i3x Multi-Mode Detection Platform. Figure 1-1: Microplate Reader and Cytometer Item Description 1 SpectraMax i3x Multi-Mode Detection Platform (microplate reader) 2 SpectraMax MiniMax Imaging Cytometer (cytometer) Note: If you are installing the SpectraMax i3x Instrument at the same time, unpack the microplate reader before setting up the cytometer, but leave the microplate reader transport locks in place until after the cytometer is attached...
- Page 4 SpectraMax MiniMax Imaging Cytometer Installation Guide Contents of the Cytometer Package The package contains the imaging cytometer and the tools and accessories required for installing the instrument as follows: Table 1-1: Cytometer Package Contents for Instrument Installation Illustration Part Number Description...
- Page 5 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Imaging Cytometer Computer System Specifications The SpectraMax MiniMax Imaging Cytometer is shipped with a computer that meets greater minimum computer system specifications than those required for the standard installation of the SoftMax Pro Software. Table 1-2: Minimum Computer System Requirements for the SpectraMax MiniMax Imaging Cytometer Item...
- Page 6 SpectraMax MiniMax Imaging Cytometer Installation Guide Unpacking the Cytometer The packaging is specifically designed to protect the SpectraMax MiniMax Imaging Cytometer during transportation. WARNING! LIFTING HAZARD. To prevent injury, use a minimum of two people to lift the instrument. Note: The shipping box and all packaging materials should be retained in case of future transport needs.
- Page 7 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Removing the Shipping Protections To prevent damage during shipping, four (4) wing nuts secure the bolts in the corners, a transport bracket with six (6) blue retaining screws and three (3) more blue retaining screws secure the optics plate on the top of the cytometer, and a cover with three (3) blue retaining screws protects the optical components in the optics tower.
- Page 8 SpectraMax MiniMax Imaging Cytometer Installation Guide This procedure requires the following tool: Table 1-4: Required Tool Illustration Part Number Description 5025413 Hex key, 3.0 mm To remove the shipping protections from the cytometer: 1. Use your hand to remove the four wing nuts (A) from the bolts attached to the corners of the instrument.
- Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51.2 in.) wide and 65 cm (25.6 in.) deep, preferably with access on all sides for lifting the instruments. The required vertical clearance for this procedure is 56 cm (22.0 in.).
- Page 10 SpectraMax MiniMax Imaging Cytometer Installation Guide To attach the cytometer to the microplate reader: 1. Place the foam pad from the shipping container on the work bench on one side of the microplate reader. CAUTION! If the SpectraMax i3x Instrument has previously been in use,...
- Page 11 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer 6. Place one of the laser-interlock brackets (H) over the optics-access opening with the interlock tab (I) on the bracket inserted into the corresponding alignment slot (J) on the bottom of the microplate reader. Figure 1-5: Laser-Interlock Brackets Item Description...
- Page 12 SpectraMax MiniMax Imaging Cytometer Installation Guide 10. Carefully place the cytometer on its side on the foam pad so that the top of the cytometer faces the bottom of the microplate reader. Note: you might need to slide the microplate reader on the foam pad to make room for the cytometer.
- Page 13 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer 12. Use a slot-head screwdriver to tighten one of the four bolts on the bottom of the cytometer. Note: You might need to use your free hand to push the cytometer into the reader to start the bolt and engage the threads.
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Page 14: Removing The Transport Locks
SpectraMax MiniMax Imaging Cytometer Installation Guide Removing the Transport Locks CAUTION! The instrument can be damaged if the transport locks are not removed before the instrument is powered on. Transport locks are placed on the detection cartridge drawer and the microplate drawer to protect the instrument from damage during shipping. - Page 15 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer To remove the transport locks: 1. Firmly pull on the front cover to remove it and then set it aside. The front cover is held onto the front of the instrument by powerful magnets. Tip: It is easiest to remove the front cover by pulling from the bottom.
- Page 16 SpectraMax MiniMax Imaging Cytometer Installation Guide 6. Replace the front cover by aligning the magnets on the inside of the front cover with the magnets on the instrument base. 7. Gently pull the yellow tab protruding from the microplate chamber door to open the door.
- Page 17 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Setting Up the System Computer The SpectraMax MiniMax Imaging Cytometer is shipped with a computer that meets the minimum computer system requirements for the instrument and the software. The computer and monitor are shipped in separate packages. Read and follow the installation instructions included with the computer and monitor.
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Page 18: Connecting The Cables
SpectraMax MiniMax Imaging Cytometer Installation Guide 13. Under Important Updates, make sure that Install updates automatically is not selected. Select one of the other options. 14. Click OK. 15. Close Control Panel. Connecting the Cables The power cords and USB cables connect to the ports on the rear of the instrument. - Page 19 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Figure 1-12: Power Switch, Fuses, and Connection Ports Item Description 1 Microplate reader USB port 2 Gas inlet quick-connect fitting 3 Power port 4 Fuse carrier 5 Power switch 6 Interconnect USB port 7 ...
- Page 20 SpectraMax MiniMax Imaging Cytometer Installation Guide Figure 1-13: Power Switch Item Description 1 Power switch 2 3 To connect the cytometer cables: 1. Make sure that the instrument and host computer are placed on a dry, flat work area with sufficient space for the two devices and the required cables. To ensure sufficient ventilation and provide access for disconnecting power from the instrument, maintain a 20 cm to 30 cm (7.9 in.
- Page 21 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer 5. Connect one end of the shorter USB cable (P) supplied with the cytometer to the interconnect USB port labeled SpectraMax® System (Q) on the rear of the cytometer, and then connect the other end of the USB cable to the microplate reader USB port (R) on the rear of the microplate reader.
- Page 22 SpectraMax MiniMax Imaging Cytometer Installation Guide 6. Connect one end of a 2 meter USB cable (S) supplied with the cytometer to a USB 3.0 port in the PCI card on the rear of the computer, and then connect the other end of the USB cable to the USB port labeled COMPUTER USB 1 (T) on the rear of the cytometer.
- Page 23 You can use the Protocol Manager in the SoftMax Pro Software to quickly find and open a predefined protocol. More protocols and updated protocols can be downloaded from the Knowledge Base on the Molecular Devices support web site (www.moleculardevices.com/support.html) or from the protocol sharing web site (www.softmaxpro.org). 5024111 C...
- Page 24 SpectraMax MiniMax Imaging Cytometer Installation Guide Installing a Detection Cartridge There are three (3) slots available in the detection cartridge drawer. You can install a detection cartridge into any available slot. To install a detection cartridge: 1. On the touch screen on the front panel of the instrument, touch the detection cartridge drawer open/close button.
- Page 25 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Figure 1-17: Top View of Detection Cartridge Drawer Item Description 1 Holder pin 2 Holder pin 3 Detection cartridge connector 4 Retaining rod 5 Retaining rod 5. Gently but firmly push the detection cartridge onto the holder pins and connector so that the detection cartridge is fully seated in the detection cartridge slot.
- Page 26 SpectraMax MiniMax Imaging Cytometer Installation Guide Figure 1-18: Detection Cartridge Retaining Clips and Retaining Rods Item Description 1 Detection cartridge 2 Retaining clip unattached 3 Retaining clip attached 4 Retaining rod 5 Retaining rod 7. Install more detection cartridges, if desired.
- Page 27 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Selecting the Light Source for Imaging The SpectraMax MiniMax Imaging Cytometer captures images from the bottom of each microplate well. You can choose to illuminate the sample with white transmitted light from the top of the microplate using the Transmitted Light (TL) Detection Cartridge, or use fluorescent excitation from the bottom of the microplate.
- Page 28 SpectraMax MiniMax Imaging Cytometer Installation Guide www.moleculardevices.com ©2015 Molecular Devices, LLC. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. All rights reserved. Specifications subject to change without notice. Patents: www.moleculardevices.com/productpatents 5024111 C FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES...
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