Molecular Devices FLIPR Tetra Manuals

Manuals and User Guides for Molecular Devices FLIPR Tetra. We have 1 Molecular Devices FLIPR Tetra manual available for free PDF download: User Manual

Molecular Devices FLIPR Tetra User Manual (302 pages)

High Throughput Cellular Screening System

Brand: Molecular Devices | Category: Laboratory Equipment | Size: 9 MB

Table of Contents
3
Chapter 1: System Overview

13
- Fluorescence Mode
  13
- Luminescence Mode
  14
- System Requirements
  14
  - Electrical
    14
  - Minimum Space
    15
Chapter 2: System Hardware Features

17
- Overview of FLIPR Tetra System Hardware Features
  17
  - System Diagram
    18
- Plate-Handling System
  18
  - Five-Position Stage
    18
  - Plates
    21
  - Instrument Status Panel
    21
  - Manual Mode
    22
  - Robotic Integration
    23
  - Observation Panel
    24
- Liquid-Handling System
  25
  - Standard Pipettor Head
    25
  - Cell Suspension
    27
  - Pin Tool Head
    29
  - Compatible Plate Configurations
    30
  - Tip and Pin Tool Loading
    31
  - Tip and Pin Tool Washing
    31
- Optical System
  32
  - CCD Camera Options
    33
  - LED Modules
    35
  - Emission Filters
    37
- Chiller
  38
- Computer System
  38
  - Host Computer
    38
  - Embedded Computer
    39
Chapter 3: Startup and Shutdown

41
- Starting up the System
  41
- Shutting down the System
  42
Chapter 4: Software Installation

43
Chapter 5: Screenworks Software Overview

47
- Screenworks Software Main Screen
  47
  - Title Bar
    48
  - Menu Bar
    48
  - Toolbar
    48
  - Status Bar
    51
- Menu Bar
  51
  - File Menu
    52
  - View Menu
    54
  - Instrument Menu
    55
  - Tools Menu
    61
  - Window Menu
    65
  - Help Menu
    65
- Instrument Status
  66
  - Instrument Status Tab
    66
  - Instrument Configuration Tab
    68
  - Process Explorer Tab
    69
- Experiment Window
  69
  - Constructing Protocols Using FLIPR Tetra Processes
    70
- Settings Process
  71
  - Setup Read Mode
    72
  - Assign Plate to Position
    74
  - Data File Name
    76
  - Folders
    76
  - Temperature Control
    77
  - Auto Print Options
    78
- Analysis Process
  79
  - Viewing Data
    79
  - Analyzing Data
    87
  - Exporting Data
    99
  - Batch Export
    106
  - Image Display
    107
  - Notes
    107
- Transfer Fluid Process
  108
- MIX Fluid Process
  117
  - MIX Fluid (Standard Pipettor)
    117
  - MIX Fluid (Pin Tool)
    119
  - MIX with TF
    120
- Read Process
  120
  - Read
    120
  - Read with TF
    122
- Wash Tips or Pins Process
  123
  - Wash Tips (Standard Pipettor)
    124
  - Wash Pins (Pin Tool)
    125
- Blot Pins Process
  126
- Pause Pipettor Process
  126
- Wash Cell Reservoir Process
  127
- Finish with Source
  128
Chapter 6: Exchanging Hardware

129
- Exchanging Pipettor and Pin Tool Heads
  129
- Exchanging the 1536 Tip Gasket
  135
  - Installing the Gasket
    135
  - Removing the Tip Block and Gasket
    136
- Exchanging Pin Tools
  137
  - Loading and Unloading the Pin Tool
    137
- Exchanging LED Modules
  138
  - Uninstalling LED Modules
    138
  - Installing LED Modules
    139
- Changing Filters
  141
  - Uninstalling a Filter
    142
  - Installing an Emission Filter
    143
- Installation of Plate Hold-Down Devices
  144
  - Selection of Appropriate Plate Hold-Down
    144
- Cell Reservoir
  146
  - Installing Cell Reservoir
    146
  - Uninstalling Cell Reservoir
    147
Chapter 7: Calibration and Signal Test

149
- Optical Calibration
  149
  - Adding a Read Plate to Plate Library
    149
  - Recalibrating the Optics
    150
- Running a Signal Test
  153
  - System Check
    153
  - Running a Plate Prior to an Experiment
    154
Chapter 8: Running an Experiment

157
- Overview
  157
- Preparing Cells for Adherent Assays
  157
  - Location of Cells in the Plate
    157
  - Cell Densities
    158
  - Cell Seeding
    158
- Preparing Cells for Suspension Assays
  159
  - Location of Cells in the Plate
    159
  - Cell Densities
    159
  - Cell Seeding
    159
- Powering-Up the System
  159
- Checking the System
  160
  - Running the Yellow Plate Signal Test
    160
- Dye-Loading the Cells for Fluorescence Assays
  161
  - Loading Duration and Temperature
    161
- Preparing a Source/Compound Plate
  162
- Setting up an Assay Protocol
  164
  - Creating a Protocol File
    164
- Optimizing the Optics and Fluid Dispensing
  165
  - Optimizing the Hardware Settings
    165
  - Optimizing Optics Hardware
    165
  - Adjusting the Pipettor Height
    167
  - Adjusting the Fluid Dispensing Speed
    168
  - Optimizing Fluid Volume
    168
  - Optimizing Pin Tool Delivery
    169
  - Optimizing Cell Delivery
    169
  - Start the Assay Run
    169
- FLIPR Calcium Assay Kit Protocol
  170
  - Required Materials
    170
  - Calcium Assay Kit Experimental Protocol
    172
  - Running the FLIPR Calcium Assay Kit
    174
  - Troubleshooting Guide
    176
- FLIPR Membrane Potential Assay Kit Protocol
  177
- Voltage Sensor Probes Assay Protocol
  184
- Luminescence Assay Protocol
  188
Chapter 9: Troubleshooting

197
- Instrument Status Colors
  197
- Troubleshooting Start-Up
  198
- General Troubleshooting
  200
- Troubleshooting the Pipettor
  205
- Troubleshooting the Optics
  210
- Troubleshooting the Yellow Plate
  215
- Troubleshooting the Tip Washer
  216
- Troubleshooting the Cell Reservoir
  218
- Troubleshooting Data
  218
- Troubleshooting Robotic Integration
  219
Appendix A: Robotic Integration

221
- Introduction
  221
  - Purpose
    221
  - Document Conventions
    221
  - Interface Versioning
    221
- Instrument Overview
  222
  - Instrument Basic Function
    222
  - Instrument Hardware Introduction
    222
  - Instrument Layout
    224
  - Plate Layout
    225
  - Plate-Handling System
    225
  - Instrument Layout Terminology (I.e., Where's the Front?)
    226
  - Robotic Plate Loading
    226
  - Optics Access Door
    227
  - Washer Placement
    227
  - Cell Suspension Placement
    228
  - Other Instrument Access Areas
    228
  - Monitor and Keyboard Placement
    230
  - Required Access Areas
    230
- FLIPR Tetra System Control Architecture
  233
  - General Description
    233
  - Manual Mode
    234
  - Remote Mode
    234
  - Communication Protocol and Address
    236
  - General Remote Mode Use
    236
- Setting up Protocols for Remote Control Use
  240
  - General Directions
    240
  - Settings Screen
    240
  - Analysis Screen
    242
  - Transfer Fluid Processes
    243
  - Wash Tips Processes
    244
  - MIX Fluid Processes
    244
  - Read Processes
    244
  - Finish with Source Processes
    244
  - Wash Cell Reservoir Processes
    244
- Command Set
  244
  - Command Syntax
    244
  - Version<Cr
    245
  - Status<Cr
    246
  - Loadplate<Tab>Location, Last Plate[, BAR CODE] <CR
    248
  - Removeplate<Tab>Location <CR
    250
  - Openprotocol<Tab>File Name<Cr
    251
  - Findprotocols<Tab>Folder<Cr
    251
  - Runexperiment<Tab>[Data File Name]<Cr
    252
  - Stopexperiment<Cr
    253
  - Clearerror<Cr
    254
  - Loadtips<Cr
    255
  - Unloadtips<Cr
    256
  - Cyclecameratemp<Cr
    257
  - Tempcontrolonoff<Tab>Temp<Cr
    257
  - Washtips<Tab>Fluid Type, Wash Cycles, Volume/Stroke, Aspirate Speed, Pump Speed, Strokes, Hold Time, Dispense Speed<Cr
    258
  - Configuration <CR
    260
  - Statusex<Cr
    262
  - Cellflaskcontrol<Tab>Rate<Cr
    264
  - Washcellreservoir<Tab>Fluid Type, Fill Speed, Drain Destination, Drain Speed, Wash Cycles, Hold Time, Volume Level<Cr
    265
- Error Handling and Reporting
  267
  - Error Types
    267
- Appendix A-Remote Interface Revision History
  
  270
  - V1.0
    270
  - V1.1
    270
  - V1.2
    270
  - V1.3
    271
- Appendix B-Bar Code Specifications
  
  271
  - About Bar Codes
    271
  - Bar Code Recommendations
    271
  - Bar Code Specifications
    272
Appendix B: Data Processing Algorithms

273
- Hypothetical Experiment
  273
- Crosstalk Correction
  274
  - Determining Crosstalk Correction
    274
- Spatial Uniformity Correction
  275
  - Determining Spatial Uniformity Correction
    275
- Negative Control Correction
  276
  - Determining Negative Control Correction
    277
- Positive Control Scaling
  278
  - Determining the Positive Control Scaling
    278
- Subtract Bias
  279
  - Determining Subtract Bias
    279
- Response over Baseline
  280
  - Determining Response over Baseline Correction
    280
Appendix C: Consumables and Accessories

283
- FLIPR Tetra System Accessories
  283
  - Field Installations
    283
  - Pipettor Heads
    283
  - Optics Consumables
    284
  - Pipetting Consumables
    285
  - Cell Reservoir Consumables
    286
- Plates
  287
  - 96-Well Read Plates
    287
  - 96-Well Read Plate Masks
    287
  - 96-Well Source Plates
    288
  - 384-Well Read Plates
    289
  - 384-Well Source Plates
    290
  - 1536-Well Read Plates
    290
  - 1536-Well Source Plates
    291
  - Source Reservoirs
    291
- Assays Performed on the FLIPR Tetra System
  291
  - Calcium Flux Consumables
    291
  - FLIPR Membrane Potential Assay Kit Consumables
    293
- Equipment and Supplies Suggested to Perform Assays with Your FLIPR Tetra System
  293
Appendix D: Using Aquamax Sterilant

295
- Principle of Use
  295
- Contents
  295
- Materials Required but Not Provided
  295
- Storage
  296
- Reagent Preparation
  296
- Reagent Use
  296
- Warnings, Precautions and Limitations
  296
Appendix E: Decontamination Certificate

299
- Procedure for Completing the Certificate
  299
Appendix F: Electromagnetic Compatibility (EMC)

301

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Molecular Devices FLIPR Tetra Manuals

Molecular Devices FLIPR Tetra User Manual (302 pages)

Table of Contents

Chapter 1: System Overview

Chapter 2: System Hardware Features

Chapter 3: Startup and Shutdown

Chapter 4: Software Installation

Chapter 5: Screenworks Software Overview

Chapter 6: Exchanging Hardware

Chapter 7: Calibration and Signal Test

Chapter 8: Running an Experiment

Chapter 9: Troubleshooting

Appendix A: Robotic Integration

Appendix A-Remote Interface Revision History

Appendix B-Bar Code Specifications

Appendix B: Data Processing Algorithms

Appendix C: Consumables and Accessories

Appendix D: Using Aquamax Sterilant

Appendix E: Decontamination Certificate

Appendix F: Electromagnetic Compatibility (EMC)

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