Molecular Devices SpectraMax Paradigm User Manual page 58

SpectraMax Paradigm Multi-Mode Detection Platform User Guide
Applications
Identify the nature of the protein or epitope effectively. Also, it can be used as a tool for
quantitative analysis of protein.
Use for chromatography components analysis, sucrose gradient analysis.
Test the endogenous or exogenous expression of phosphoprotein so as to detect the
phosphorylation signal.
Protein resilience in the function experiment.
Structure domain analysis.
Analysis of the protein expression level.
Analysis of protein content in the serum.
Analysis of regulatory proteins expressed in the cell cycle.
You can use the Protocol Manager in the SoftMax Pro Software to quickly find and open a
predefined protocol.
More protocols and updated protocols can be downloaded from the Knowledge Base on the
Molecular Devices support web site (www.moleculardevices.com/support) or from the
protocol sharing web site (www.softmaxpro.org).
The following detection cartridge has Western Blot TRF read mode capability:
ScanLater Western Blot (WB) Detection Cartridge
The ScanLater Western Blot (WB) Detection Cartridge can be used for top reads only.
Analyzing Western Blot TRF Data
After scanning a membrane for Western Blot data, the data are displayed in the SoftMax Pro
Software as an image. You can use the image tools in the Plate section to zoom, crop,
colorize, and adjust the intensity of the image. You can also select a region of interest (ROI)
and rescan the membrane at a higher resolution.
Western Blot membrane data are saved as a TIFF image that can be analyzed by the image-
analysis tool of your choice. The SoftMax Pro Software comes with an installed version of the
ImageJ software from U.S. National Institute of Health (NIH).
For best results, use the Molecular Devices ScanLater™ Western Blot Assay Kit that matches
your application.
To ensure successful acquisition of Western Blot data, see the following topics:
Blocking Nonspecific Binding on page 59
Handling the Membrane on page 59
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