Rdw Calibration; Rdw Calibration Using A Quality Control Standard - HORIBA ABX Micros 60 User Manual

2.2.10. RDW Calibration

The RDW calibration is a separate calibration outside the Auto-calibration menu.
The RDW calibration coefficient default value is normally set at 1.00.
RDW coefficient editing
Main Menu
3- Calibration
2- Coefficients
1- Calib. Coeff./ password 123 or the one defined by the operator in the setup menu.
(See Section 5, 4.2. Changing (the User) Password
Press «Enter» key
Select the # 7 key or use the «Down» arrow key to select the RDW coefficient
Press «Enter» key to edit the coefficient
Enter the RDW coefficient value which has been calculated from the comparison study.
Press «Enter» key to accept the new value
Return to Main menu pressing the «Esc» key
The RDW calibration is normally performed by taking blood samples from 100 healthy,
normal, and drugfree individuals. These blood samples are then analyzed on instrument
that has been calibrated for RDW determination. The Mean and Standard Deviation are
then calculated from that population analyzed. The same 100 samples are then analyzed
on the ABX Micros 60. A population Mean is calculated and then compared to
the known calculated Mean from the comparison instrument. The RDW calibration coef-
ficient for the ABX Micros
Mean values.
Expected RDW values may vary with sample population and/or geographical location. it
is highly recommended that each laboratory establish its own normal ranges based on
the local population.

2.2.11. RDW calibration using a Quality Control Standard

The RDW may also be calibrated by using a known Quality Control Standard.
DEFINITION: A Quality Control Standard is defined as a Commercial blood product which
has been specifically developed and Assayed with set parameter Target values and rang-
es. This product is designed to precisely measure the accuracy and linearity of the ana-
lyzer.
1- Take the controls and bring them to room temperature. Gently and thoroughly mix the control
material as indicated on the instruction sheet that comes with the control kit.
2- Run the Normal Level control 6 times, as a regular patient analysis. When the cycles are complete,
note only the RDW results. Write down these results for future reference use.
3- Calculate the Mean value for all 6 results and write it down for future reference use.
4- Take the Control Assay sheet that comes with the control kit and note «Only», the Normal Control
Mean Assay value for RDW.
Calculate the new RDW coefficient as followed:
5- Take the Normal Control Mean Assay value for RDW, Divide it by the Mean value of the Normal
control ran 6 times as a sample, times the current RDW calibration coefficient, This will equal the
New RDW Calibration coefficient.
6- Proceed as described in " RDW coefficient editing, page 17"
Quality
, page 5-10).
60 is then calculated from the difference of the two
to enter this new value.
ABX Micros 60 - User Manual - RAB042FEN - 17
Assurance
Calibration