Hgb (Hemoglobin); Mcv (Mean Corpuscular Volume); Mch (Mean Corpuscular Hemoglobin); Mchc (Mean Corpuscular Hemoglobin Concentration) - HORIBA ABX Micros 60 User Manual

ABX Micros

4.3.4. HGB (Hemoglobin)

Turbidity of the Blood sample - Any number of physiologic and/or theraputic factors may produce
falsely elevated Hemoglobin results. To obtain accurate HGB results when increased turbidity of the
blood sample occurs, determine the cause of the turbidity and follow the appropriate Method below:
1- Elevated WBC: An extremely elevated WBC will cause excessive light scatter from the L.E.D. In
these cases, use Reference (Manual) methods. The Diluted sample should be Centrifuged, and the
Supernatant fluid measured with a Spectrophotometer.
2- Elevated Lipids: Elevated Lipids in the blood will give the plasma a «milky» appearance. This
condition can occur with Hyperlipidemia, Hyperproteinemia(as in gammapathies), and Hyperbiliru-
binemia. Accurate Hemoglobin measurement can be achieved by using reference (Manual) methods
and a plasma blank
Increase in Turbidity may also be seen in cases where the Red blood cells are resistant to the Lysing
action. This condition will cause a falsely elevated HGB result, but may be detected by observing
the abnormal MCH and MCHC values, and the increased baseline on the (Left leading edge) of the
WBC Histogram. Erroneous HGB results will cause the results of the MCH and MCHC to be erroneous
as well.
Fetal Bloods - The mixing of fetal and maternal bloods may produce a Falsely elevated Hemoglobin
value.

4.3.5. MCV (Mean Corpuscular Volume)

Red Blood cell Agglutination - May produce an erroneous MCV value. Red blood cell agglutination
may be detected by observing abnormal MCH and MCHC values, as well as examination of a stained
blood smear. In such cases, Manual methods may be required to obtain an accurate MCV value.
Excessive Numbers of Large Platelets - and /or the presence of an excessively High WBC count may
interfere with the accurate determination of the MCV value. Careful examination of a stained blood
smear may reveal the error.

4.3.6. MCH (Mean Corpuscular Hemoglobin)

The MCH is determined, according to the HGB value and the RBC count. The Limitations listed for
HGB and RBC will have an effect on the MCH and may cause erroneous values.

4.3.7. MCHC (Mean Corpuscular Hemoglobin Concentration)

The MCHC is determined, according to the HGB and HCT values. The Limitations listed for HGB and
HCT will have an effect on the MCHC and may cause erroneous values.

4.3.8. RDW (Red cell Distribution Width)

The Red blood cell distribution width is determined, according to the RBC count. The red blood cells
pass through a Micro-aperture that will generate electronic pulses, as the cells pass through it. These
pulses are then Grouped according to size, Thresholded, and calculated to form a Histogram (Distri-
bution curve). This distribution curve is then used to calculate the distribution of the Red blood
cells as a percentage of the curve. This curve is then used in determining the RBC size abnormalities
as in Anisocytosis.
Agglutinated Red Blood cells - May cause a falsely low RBC count and erroneous RDW's. Blood sam-
ples containing the agglutinated RBC's may be detected by observing abnormal MCH and MCHC
values, as well as examination of a stained blood smear.
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ABX Micros 60