Hgb - HORIBA ABX Micros 60 User Manual

Number of cells
Fig.2-4: RBC Pulses electronically calculated and smoothed: RBC Distribution curve
Number of cells
Fig.2-5: PLT pulses electronically calculated and smoothed: PLT distribution curve
RBC Histogram: is an electronic Distribution and mathematical calculation of the RBC's placed into
256 Channels of volumetric sizing from 30fl to 300fl.
PLT Histogram: is an electronic distribution and mathematical calculation of the PLT's placed into
128 channels of volumetric sizing from 2fl to a mobile threshold between the high end platelets to
the Low end Red Blood Cell Thresholds.
(fl = fentaliters) A microscopic volumetric unit of measurement. This is a 3-dimentional measurement
used to determine the volume of Microscopic particles.

2.3.2. HGB

The Hemoglobin measurement is based on a Startup cycle. This cycle includes a Hemoglobin Blank
test sequence which includes 2 Hemoglobin blank measurements. Each analysis cycle run after Start-
up also has a Hgb Blank measurement which is compared to the initial Start-up Hgb Blank. Each
analysis cycle run thereafter compares the Hgb Blank reading to the previous cycle Hgb Blank read-
ing.
During the WBC analysis cycle, 0.52ml of Lyse Reagent is added to 2.05ml of diluted blood in the
WBC Chamber. The Lyse reagent contains potassium ferricyanide [Fe(Cn)]K, and potassium cyanide
[KCN]. This lysing reagent breaks down the RBC Cell membrane and releases the Hemoglobin within
the RBC.
The Hemoglobin then combines with the potassium cyanide to form a chromogenous cyanmethemo-
globin compound. This chemical compound is measured by Spectrophotometry, through the optical
pathway in the WBC chamber. The light wavelength of measurement is at 550nm.
HGB using ABX Lysebio : Reagent for erythrocyte lysis and cyanide-free determination of hemoglo-
bin. All the heme iron is oxidized and stabilized producing chromogenic species for quantitation by
spectrophotometry at a wavelength of 550nm.
Results, The Hgb results are given as such:
HGB = Log(blank value/Sample value) x the Calibration Coefficient.
Description &
Cell size
Cell size
ABX Micros 60 - User Manual - RAB042FEN - 7
Technology
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