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2. Calibration
2.1. General recommendations
The calibration on HORIBA ABX instruments is an exceptional procedure, which must be carried out
particularly in case of certain technical interventions (installation, maintenance, service interven-
tion). The calibration should not be carried out to compensate a drift on a result due for example
to a clogging of the instrument.
2.1.1. Preliminaries
Before carrying out a calibration, it is essential to make sure that the instrument is in perfect con-
dition of operation, and to follow the following steps:
1-
Carry out a Concentrated cleaning procedure see (See Section 6,
page 6-11).
2-
Perform two blank cycles to check the cleanliness of the instrument.
3-
Check the repeatability of the instrument by running 6 times a normal human blood without tak-
ing account of the first result.
4-
Check that the CV obtained on the other 5 results are lower than: WBC: 2%, RBC: 2%, HGB: 1%,
HCT: 1%, PLA: 5%
5-
Run a control blood and check that the values are within the acceptable limits. If not, run a new
control blood.
If the values are still out of the acceptable limits and instrument is clean (blank cycles in conformity
with the values given in the manual) and repeatability is correct (Acceptable CV values), carry out the
calibration as described in
"2.1.2. Calibration procedure, page
2.1.2. Calibration procedure
1-
Run at least 4 times Calibrator without taking the values of the first result into account (see
"2.2.5. Running calibrator , page
2-
Calibrate the instrument on the average of the last three results according to indications of the
manual See
"2.2.8. Calibration Passed , page 15"
3-
Run 3 times again Calibrator to check the values.
4-
Confirm the calibration while passing a control blood, the values have to be returned within ac-
ceptable limits.
5-
After about thirty numerations analyses of the day, check that values of MCV, MCH and MCHC are
in conformity with the usual values of the laboratory.
2.2. Calibration function
The
ABX Micros
60
1-
Calibration is performed using a Calibrator Blood sample.
2-
Known Calibration Coefficients can be directly entered by selecting a Calibration Menu option.
13").
Calibration can be achieved in 2 different ways.
ABX Micros
Quality
Assurance
1.3.6. Concentrated
Cleaning,
11".
60
- User Manual - RAB042FEN - 11
Calibration
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