Plt (Platelets); Mpv (Mean Platelet Volume) - HORIBA ABX Micros 60 User Manual

Nutritional Deficiency or Blood Transfusion - May cause elevated RDW results due to Iron, Vitamin
B12, or Folate conditions. High RDW's may also be present from Bi-modal RBC distribution from
Transfused Blood. This will be detected by the RBC Histogram showing 2 distinctive peaks on the
distribution curve.

4.3.9. PLT (Platelets)

Very Small Erythrocytes - Microcytes, Erythrocyte's fragments - Schistocytes, and WBC fragments
may interfere with the proper counting of Platelets, and cause elevated Platelet counts.
Agglutinated Red Blood cells - May trap platelets, causing an erroneously Low platelet count. The
presence of agglutinated RBC's may be detected by observing abnormal MCH and MCHC values, and
by careful exmination of a stained blood smear.
Giant Platelets in Excessive Numbers - May cause an erroneously low platelet count since these
Large platelets may exceed the Upper Threshold limit for platelets, and are not counted as platelets.
Chemotherapy - Cytotoxic and Immunosuppressive drugs may increase the fragility of these cells
which may cause Low platelet counts. Reference (Manual) methods may be necessary to obtain an
accurate platelet count.
Hemolysis - Hemolyzed specimens contain Red blood cell Stroma which may cause elevated platelet
counts.
A.C.D. Blood - Blood anti-coagulated with Acid-Citrate-Dextrose may contain Platelet Aggregates
which could give falsely low platelet counts.
RBC Inclusions - Erythrocyte inclusions such as Howell-Jolly bodies, Heinz bodies, Siderotic and Ba-
sophilic granules,........etc, may produce considerably elevated platelet counts.
Platelet Agglutination - Clumped Platelets due to poor collection techniques or platelet satellitosis
caused by EDTA activation of Immunoglobulins may cause a Low platelet count and/or an elevated
WBC count.
These types of specimens should be re-collected in Sodium-citrate anticoagulant and re-analyzed
«For Platelets Only»!

4.3.10. MPV (Mean Platelet Volume)

Giant Platelets - that exceed the upper thresholding of the platelet channels, may not be counted
as platelets. consequently, these larger platelets will not be included in the instrument's calculations
of the mean platelet volume.
Blood Samples collected in EDTA will not maintain a stable Mean Platelet Volume. Plate-
lets collected in EDTA will swell with time and temperature
Agglutinated Red Blood cells - May trap Platelets, causing an erroneous MPV result. The presence
of Aggultinated erythrocytes may be detected by observing abnormal MCH and MCHC values, and by
careful examination of a stained blood smear.
Very Small Erythrocytes - Microcytes, Erythrocyte fragments - Schistocytes, and White Blood cell
fragments may interfere with the proper sizing and counting of the Platelets.
Chemotherapy - May also affect the Sizing of Platelets.
ABX Micros 60 - User Manual - RAB042FEN - 17
Specifications
Limitations