Running Calibrator; Calibration Results - HORIBA ABX Micros 60 User Manual

Hematology analyzer
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Changing sample number
«Change sample # ?» message is displayed
Press the «Enter» key
Enter the number of samples you wish to run for calibration or press the «Esc» key.
The number of samples you can run for calibration is a Minimum of 3 and a Maximum
of 11. In order for the instrument to provide the best mathematical data for a good cal-
ibration, a Minimum of 6 sample runs is highly recommended for quality statistical cal-
ibration data.

2.2.5. Running calibrator

From the previous screen (See
follows to run an analysis on calibrator:
Running calibrator sample
«Run Cal ?» message is displayed
Press the «Enter» key (a prime cycle is launched first)
1-
Now gently and thoroughly mix the Calibrator material as indicated on the Instruction sheet that
comes with the calibrator.
2-
Place the calibrator beneath the sample probe, and raise the vial upwards so that the sample
probe enters into the blood.
3-
Press the «Start» key on the front panel to initiate the cycle of the first sample, or press the man-
ual sample bar. The cycle will begin and the sample will be aspirated.
Wipe any excess blood from the cap and threads of the calibrator vial with a lint-free
tissue to prevent any dried blood from reentering into the calibrator material. Dried
Blood re-entering into the vial may give error flags and reject the sample runs.

2.2.6. Calibration Results

Validating results
Verify that the results are within 20% of each parameter target value.
Press the «Enter» key to continue.
«Valid calibration #1/6» message is displayed
Press «Enter» to valid («Esc» to reject and to return to the first run)
«Start calibration #2/6» message is displayed
Calibration results having Error flags such as ( $, *, or ! for HGB) will automatically be
rejected! The system will automatically re-set itself to rerun that sample. If you acquire
3 rejects continuously on the same number sample, abort calibration and contact your
local HORIBA ABX Technical Support representative!
4-
Place the cap back onto the vial, gently and thoroughly mix the material for the next calibration
sample run.
5-
Run the remaining calibrator samples, as described in
When the last sample result has been validated, the
calibration factors for each parameter. These statistical calculations include the Mean, Target, Coef-
ficient of Variation, Percent difference between the Target value and the Mean, Previous Calibration
coefficients, and the New Calibration Coefficients. The Status will indicate on the printout if a pa-
rameter has Passed or Failed Calibration.
"2.2.4. Change number of calibration samples , page 12"
"2.2.5. Running calibrator , page
ABX Micros
ABX Micros
Quality
Assurance
) proceed as
13".
60
calculates the statistical
60
- User Manual - RAB042FEN - 13
Calibration

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