Pdw; Wbc - HORIBA ABX Micros 60 User Manual

2.3.8. PDW

The PDW (Platelet Distribution Width) is calculated from the Platelet Histogram/ Distribution curve.
The PDW is represented by the width of the curve between 15% of the number of platelets starting
from the Low Threshold 2fl (S1) and 15% of the number of platelets begining with the Variable High
Threshold (S2). The PDW determination area is shown by the distribution curve below.
S1
Fig.2-6: Platelet distribution curve
PCT and PDW are not available in the United States. These parameters are strictly used
for research and investigational purposes «Only».

2.3.9. WBC

The WBC Measurement principles are based upon the same as the RBC/PLT measuring principles. The
WBC count is performed in the WBC/HGB Chamber. The electronic signal processing device places
and electronic threshold between the WBC's and the PLT's. The electronic pulses are then placed into
256 Channels according to pulse size. The pulses are then Thresholded, grouped, then mathemati-
cally calculated to create a numerical value for the determination of WBC's.
2.3.9.1. WBC Histogram
The WBC Histogram is a distribution study that reveals the following 3 types of White blood cell sub-
populations: (Lymphocytes, Monocytes, and Granulocytes). The Diluent and Lyse of the
cros 60
play a very important part when sizing the WBC subpopulations in the WBC
distribution curve.
2.3.9.2. Differential Measuring principles
◆Diluent and Lysing action:
The Diluent preserves and prepares the WBC cell membrane for the differential reaction. The Lysing
reagent has a differential mode of action on the WBC cytoplasmic membranes.
When the Lyse reacts with the Lymphocyte cytoplasmic membrane, it allows the release of water
soluble cytoplasm and shrinks the membrane around the nucleous.
When the Lyse reagent reacts with the Monocyte cytoplasmic membrane, it has an intermediate re-
action which keeps the cell somewhat stable, maintaining its large size in comparison to the
lymphocytes.
Description &
S2
ABX Micros 60 - User Manual - RAB042FEN - 9
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