Cbc Measurement Principles; Rbc/Plt - HORIBA ABX Micros 60 User Manual

Tab.2-5: RBC/PLT measurement
RBC/PLT measurement
Count Vacuum
Count period
Temperature reaction
28.3µl of diluted blood is aspirated from the WBC chamber. The needle carriage assembly moves to
the right over the RBC/PLT chamber.
2.0ml of Diluent and the 28.3µl of diluted blood is injected into the RBC/PLT chamber from the inner
sample probe.
0.5ml of Diluent is added to the dilution in the chamber from the outer sample probe. This makes
the total dilution for the RBC/PLT.
Diluent
Lyse
Sample
Fig.2-1: sample dilution

2.3. CBC Measurement Principles

2.3.1. RBC/PLT

The RBC's and PLT's are measured by an electronic impedance variation principle. This means that
an electronic field is generated around the micro-aperature in which the blood cells pass through.
The cells create a resistance in the electronic field as they pass through the Calibrated micro-aper-
ture. This inturn causes an electronic pulse to be generated which is amplified, measured, and then
mathematically calculated to create a numerical value.
First the 28.3µl Diluted Blood sample is diluted in an Electrolytic Diluent (electronic current con-
ducting fluid) mixed, then pulled through a calibrated micro-aperture. There are two electrodes that
are placed on each side of the aperture and a constant electronic current passes between them.
Description &
-200mb
2 x 5 seconds
ambient
RBC
WBC
PLT
HGB
ABX Micros 60
Technology
Technology
- User Manual - RAB042FEN - 5