Dilutions; Wbc/Hgb; Rbc/Plt - HORIBA ABX Micros 60 User Manual

ABX Micros

2.2. Dilutions

2.2.1. WBC/HGB

Tab.2-1: WBC/HGB Dilution
WBC/HGB Dilution
Initial blood volume
Volume of ABX Minidil LMG
Volume of ABX lyse
Final dilution ratio
Tab.2-2: WBC Measurement
WBC Measurement
Method
Aperture diameter
Count Vacuum
Count period
Temperature reaction
Tab.2-3: HGB measurement
HGB measurement
Wavelength
Count period
Temperature reaction
10µl of blood sample contained in the sample probe is pushed with 1.2ml of ABX Minidil LMG along
with 0.5ml of ABX Minidil LMG from the outer probe into the WBC/HGB Chamber.
0.4ml of ABX Minidil LMG is added the WBC/HGB Chamber making the diluent volume 2.1ml.
0.52ml of ABX lyse is added to the mixture.
This makes the total dilution for WBC/HGB.

2.2.2. RBC/PLT

Tab.2-4: RBC/PLT dilution
RBC/PLT dilution
Initial Diluted blood volume
Volume of ABX Diluent
Final dilution ratio
Tab.2-5: RBC/PLT measurement
RBC/PLT measurement
Aperture diameter
4 - RAB042FEN - User Manual -
60
10.0µl
2.1 ml
0.52 ml
1/260
Impedance
80 µm
-200mb
2 x 5 seconds
ambient
550nm
2 x 5 seconds
ambient
28.3µl
2.5ml
1/15 000
50µl
ABX Micros 60