Type B2 Supply Hepa Filter Leak Test Preparation; Theoretical Upstream Concentration; Upstream Concentration Sampling - Labconco Logic+ A2 Technical Manual

Type B2 Supply HEPA Filter Leak Test Preparation

All biosafety cabinet models were tested to the HEPA Filter Leak Test as described in
the current NSF/ANSI Standard 49. This section describes the methods, specifications,
and Pass/Fail criteria to challenge the supply HEPA filter in the biosafety cabinet.
You never know what a biosafety cabinet has been exposed to, so it is always
recommended to surface decontaminate any components prior to working inside the
biosafety cabinet and/or removing components from the biosafety cabinet.
1. Remove the work surface by lifting the handles on either end. Pull the work
surface straight out of the biosafety cabinet.
2. Turn the blower of the biosafety cabinet on.
3. Check the oil level of the aerosol generator is within 1/8 inch (3 mm) of the level
line.
4. If the aerosol generator requires pressurized air, connect the air line.
5. Verify the generator's air pressure is 23 psi.
6. Turn on the photometer and allow it to operate for a minimum of 5 minutes.
Leave the valve in the "CLEAR" setting.

Theoretical Upstream Concentration

If you do not wish to connect the Upstream Sampling Tube to the photometer to
establish the actual 100% concentration value, the theoretical value will need to be used
instead. Testing at Labconco has shown the actual concentration varies from the
theoretical value. Table 10-2 shows the actual vs. calculated concentrations for Type B2
models. Establish the 100% and 0% concentration levels for the photometer using the
actual values provided in Table 10-2 for the model being tested.

Upstream Concentration Sampling

You never know what a biosafety cabinet has been exposed to, so it is always
recommended to surface decontaminate any components prior to working inside the
biosafety cabinet and/or removing components from the biosafety cabinet.
1. Locate the Upstream Sample Tube on the right side, protruding from the rear
baffle. See Figure 10-9. On some models this tube is clear.
2. Pull the Upstream Sample Tube forward. Do not remove the cap yet.
3. Place the aerosol generator on top of the biosafety cabinet, see Figure 10-10.
4. Remove the inlet pre-filter (Figure 10-10).
5. When prompted by the photometer, turn on the appropriate number of Laskin
nozzles on the aerosol generator (see Table 10-2), uncap the Upstream
Sampling Tube and sample the upstream concentration to establish the 100%
concentration level.
6. Establish the 0% concentration level according to your photometer's instructions.
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