Type B2 Exhaust Hepa Filter Leak Test Preparation; Theoretical Upstream Concentration - Labconco Logic+ A2 Technical Manual

Type B2 Exhaust HEPA Filter Leak Test Preparation

All biosafety cabinet models were tested to the HEPA Filter Leak Test as described in
the current NSF/ANSI Standard 49. This section describes the methods, specifications,
and Pass/Fail criteria to challenge the exhaust HEPA filter in the biosafety cabinet.
You never know what a biosafety cabinet has been exposed to, so it is always
recommended to surface decontaminate any components prior to working inside the
biosafety cabinet and/or removing components from the biosafety cabinet.
1. Remove the work surface by lifting the handles on either end. Pull the work
surface straight out of the biosafety cabinet.
2. Turn the blower of the biosafety cabinet on.
3. Place the aerosol generator in the left, rear corner of the biosafety cabinet's drip
pan, see Figure 10-15. Ensure that the generator is level, and the oil level is
within 1/8 inch (3 mm) of the level line.
4. If the aerosol generator requires pressurized air, connect the air line.
5. Verify the generator's air pressure is 23 psi.
6. Turn on the photometer and allow it to operate for a minimum of 5 minutes.
Leave the valve in the "CLEAR" setting.

Theoretical Upstream Concentration

Actual upstream sampling is not possible on a Type B2 exhaust filter, so the theoretical
concentration will be used. Testing at Labconco has shown the actual concentration
varies from the theoretical value. Table 10-3 shows the actual vs. calculated
concentrations for Type B2 models. Establish the 100% and 0% concentration levels for
the photometer using the theoretical values provided in Table 10-3 for the model being
tested.
Figure 10-15
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