Type B2 Exhaust Hepa Filter Leak Test; Acceptance Criteria - Labconco Logic+ A2 Technical Manual

Type B2
Height
Cabinet Width
(inches)
4-foot
6-foot
1. The calculated concentration was established using the formula:
Concentration (ug/l) = (# Laskin nozzles @ 23 PSIG x 13,500) / Volume of air displaced
2. Based on Mineral oil

Type B2 Exhaust HEPA Filter Leak Test

During this test the exhaust HEPA filter will be challenged and a downstream average
utilized to check for leaks. This section describes how to test the exhaust HEPA filter
on a Type B2 biosafety cabinet. The aerosol generator and photometer should be
prepared at this point as described in the preparation steps listed in the previous
section.
1. Locate a sampling port downstream of the exhaust HEPA filter. The sampling
port is not part of the biosafety cabinet. It must be facility supplied as part of the
facility's exhaust system ducting. The sampling port should ideally be at least 10
duct diameters downstream of the exhaust HEPA filter, and the same distance
from any elbows in the duct work.
2. Connect the sampling port to the photometer.
3. Set the photometer sampling valve to "DOWNSTREAM". Ensure proper vacuum
at the sampling tube.
4. Open the appropriate number of Laskin nozzles on the aerosol generator (see
Table 10-3).
5. Observe the average concentration of aerosol downstream.

Acceptance Criteria

Aerosol penetration shall not exceed 0.005%.
Reinstall the work surface.
Sash Exhaust Air
Displacement
(CFM)
8 723
8 1083
Laskin Calculated
Nozzles Concentration
Required
2
2
65
Table 10-3
Actual
Concentration
1
(ug/L)
(ug/L)
38 21
25 17
2