Agilent Technologies 1290 Infinity II 2D-LC User Manual page 203

8 Run the System
Run the Experiment
Table 16 Recommended conditions in 2D (HPLC) for standard heart-cutting
Parameter Value
2D Gradient: Analysis 1.25 min, Equilibration 0.50 min
Analytical gradient - Shifted Gradient Shift 1D:
10 % B 0.00 min -
60 % B 1.25 min
Flush gradient not used
2
D Detector (DAD)
Diode-array 254 nm, Bandwidth 4 nm
Reference Wavelength 360 nm
Reference Bandwidth 100 nm
Peak width 80 Hz
Stop time As pump/No limit
2D-LC User Guide
30 % B 20 min
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