Agilent Technologies 1290 Infinity II 2D-LC User Manual page 269

9 Data Analysis
GC Image Basic Information
Investigate the effects of using different gradients in
When combining separation systems with related separation mechanisms in the
first and second dimension (as in RPxRP), orthogonality is limited. As a result,
only a part of the available two-dimensional separation space will be occupied. In
such a case, shifted gradients in the second dimension can be used to enlarge
the accessible two-dimensional separation space.
1 To investigate the effects of using different gradients in the second
2D-LC User Guide
dimension, firstly run a comprehensive 2D-LC separation with the same
second dimension gradient from 5 – 95 % B repeated during the whole run.
1
The D pump method should be set up as during the checkout runs (see
below):
2
D
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