Agilent Technologies 1290 Infinity II 2D-LC User Manual page 202

8 Run the System
Run the Experiment
Table 16 Recommended conditions in 2D (HPLC) for standard heart-cutting
Parameter Value
2D-LC Valve
SHC or MHC with 40 µl sample, Transfer Capillary, ASM Factor No
2
D Column Compartment (MCT)
Column RRHD Bonus-RP, 2.1x 50 mm, 1.8 µm, 1200 bar (857768-901)
Column temperature 40 °C
Stop time As pump/No limit
2
D Pump
Heart Cutting (time or peak based)
Mobile Phase A Water + 0.2 % formic acid
Mobile Phase B Methanol
Flow Rate 1.0 mL/min
Idle flow not used
Stop time 40 min (will not automatically prolonged, if peaks in 2D are not work off)
Post time 6 min
Sampling Table Start 4.08 min, minimum 1 cut required (time based or peak based), Cut Size 4.0
Figure 105 Time-based
Figure 106 Peak-based
The Cut-Time (SHC) can vary slightly depending on the configuration and the used hardware.
2D-LC User Guide 202