Agilent Technologies 1290 Infinity II 2D-LC User Manual page 212

8 Run the System
Run the Experiment
Table 23
Parameter
Ion Source
Ion Mode
Ion polarity
Storage Mode
LCMS Stream
Acquisition Mode
Gas Temp
Gas Flow
Nebulizer
SheathGasTemp
SheathGasFlow
Scan Segment
Vcap
Nozzle Voltage
Fragmentor
Skimmer1
OctopoleRFPeak
Ref Mass Enabled
Use Bottle A
RefNebulizer
Ref Nebulizer
Average Scans
Detection Window
(ppm)
2D-LC User Guide
Recommended conditions in 2D (LC-MS)
Value
Atmospheric pressure electrospray (Dual AJS ESI)
Dual AJS ESI
Both
Both
MS
Acquisition Mode MS1 Min Range (m/z) 50, Max Range (m/z) 1000, Scan
Rate (spectra/sec) 3.00
Instrument Parameters
250 °C
11 L/min
40 psig
350 °C
12 L/min
1
Scan Source Parameters
3500 V
300 V
120
45
750
ReferenceMasses
Enabled
True
0 psig
AutoRecalibration
1
100 ppm
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