Agilent Technologies 1290 Infinity II 2D-LC User Manual page 97

4 Installation
Software Installation
[OPTIONAL] 1 To change the Device Name, connection settings and the Pressure Units, fill in
2 To verify the correct
This action will not rename your pumps. Enter a descriptive naming during initial
NOTE
instrument setup in the instrument configuration, see
Instrument"
The Configure 2D Pump button allows the configuration of the
example, the solvent types.
3 Select
This action will not rename your detectors. Enter a descriptive naming during
NOTE
initial instrument setup in the instrument configuration, see
Instrument"
To determine the transfer volume (
NOTE
more precisely, you can run a time-based High-Resolution experiment (multiple
cuts) over one of the first sample peaks. The cut with the highest abundances
then corresponds to the apex of your peak. if there is a shift of the peak to the
front or to the back, the difference in volume can be calculated and the transfer
volume adjusted.
Up to four CAN capable detectors are supported in each dimension.
NOTE
2D-LC User Guide
the according fields.
1
settings.
on page 95.
1 2
D and D detector under Detectors.
on page 95.
If necessary, it is possible to configure and select more than two detectors,
for example, an LC/MS Single Quadrupole.
1
The D settings for the transfer volumes that determine the time between the
1
D detection of the peak and the switching of the 2D-LC Valve, depends on the
hardware setup. For a standard 2D-LC with two DADs, the transfer volume is
approx. 14 µL.
To calculate the volume, add half the volume of the detector flow cell plus the
volume between the detector flow cell and the 2D-LC Valve.
The transfer volume possibility for the
between the 2D-LC Valve and the second dimension detector flow cell. This
result is set for delay calculation in 2D-LC OpenLab DA-Plugin.
between Sample Loop and detector ensures, that start and end of a cut
chromatogram are aligned/displayed correctly.
D and 2D pump configuration, check the Pumps
2
D detector defines the volume
1
D Detector to 2D-LC Valve) experimentally
"Configure the HPLC
2
D pump like, for
"Configure the HPLC
2
D offset
97