Agilent Technologies 1290 Infinity II 2D-LC User Manual page 209

8 Run the System
Run the Experiment
Table 20
Parameter
Nozzle Voltage
Fragmentor
Skimmer1
OctopoleRFPeak
Ref Mass Enabled
Use Bottle A
RefNebulizer
Ref Nebulizer
Average Scans
Detection Window
(ppm)
Min Height
Stop time
1 Load method Multiple Heart-Cutting Checkout from the 2D-LC data media and
2 Run the method with 2D-LC starter sample, 1 x 2 mL (5190-6895), 1:10 (for
3 If necessary, subsequently edit or optimize the method.
2D-LC User Guide
Recommended conditions in 2D (LC-MS)
Value
300 V
120
45
750
ReferenceMasses
Enabled
True
0 psig
AutoRecalibration
1
100 ppm
1000 counts
Reference Masses
Positive
121.05087300
922.00979800
Chromatograms
Chrom Type Label Offset Y-Range
TIC TIC 1510000000
TIC TIC 1510000000
As pump/No limit
modify the settings for your multiple heart-cutting configuration.
only UV Checkout), 1:100 (for LCMS Checkout), or 1:1000 (for LCMS
Checkout) diluted with Methanol/Water (20/80; v/v) with 0.1 % formic acid.
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