Agilent Technologies 1290 Infinity II 2D-LC User Manual page 113

5 2D-LC Data Acquisition in MassHunter Workstation 11
2D-LC User Interface
Transfer Volume
The
detection of the peak and the switching of the 2D-LC valve, depend on the
hardware setup. For a standard 2D-LC with two DADs, the transfer volume is
approx. 14 µL. To calculate the volume, you have to add half the volume of the
detector flow cell plus the volume between the detector flow cell and the 2D-LC
valve.
If a second
NOTE
detectors in the first dimension volume must also be entered in the signal
selection of the reference chromatogram see
Transfer Volume
The transfer volume possibility for the
the 2D-LC valve and the second dimension detector flow cell. This is the result
set for delay calculation in 2D-LC OpenLab DA-Plugin.
loop and detector ensures, that start and end of a cut chromatogram are
aligned/displayed correctly.
For the MassHunter workflow with a High-End mass spectrometer as additional
NOTE
second detector, you have to define this transfer volume (delay) during the file
splitting setup in the data evaluation.
2D-LC User Guide
1
D detector
1
D settings for the transfer volumes that determine the time between the
1
D detector is installed, the transfer volume between the two
2
D detector
"Method Parameters"
2
D detector defines the volume between
2
D offset between sample
1
D
on page 125.
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