Agilent Technologies 1290 Infinity II 2D-LC User Manual page 221

8 Run the System
Run the Experiment
Table 29 Recommended conditions in 2D (HPLC) for ASM MHC 2D-LC
Parameter Value
Sampling Table Start 0.2 min Time based, 2.5 min Peak Based, 4.50 min
2D Gradient Analysis 1.50 min Equilibration 0.50 min Cycle time 2.12 with ASM ON and ASM Factor 3
Analytical gradient - Shifted Gradient Shift 1D:
3 % B 0.00 min
3 % B 0.37 min
10 % B 0.38 min
60 % B 1.62
2
D Detector (DAD)
Diode-array 254 nm, Bandwidth 4 nm
Reference Wavelength 360 nm
Reference Bandwidth 100 nm
Peak width 80 Hz
Stop time As pump/No limit
2D-LC User Guide
30 % B 6 min
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