Agilent Technologies 1290 Infinity II 2D-LC User Manual page 140

6 Method Parameters
Set the 2D-LC Method parameters
Use Loop Flushing and Active Solvent Modulation (ASM)
If your 2D-LC instrument is equipped with the G4236A 2D-LC ASM Valve, this
method development feature helps finding the optimal dilution of
the sample loop. ASM leads to best
ASM settings of 2D-LC method parameters allow switching on and off the use of
the ASM functionality.
•
•
1 To use ASM, mark the ASM check box.
For visual verification of the ASM phase, you can check the Analytical Gradient
NOTE
Graph. There you can see the impact of the ASM phase before the
The gradient with ASM increases the cycle/modulation time.
To change the ASM factor you must install and configure the new ASM capillary
NOTE
in the 2D-LC UI, see
There are four different ASM capillaries available. You can find more info for
Installation and Configure of different ASM capillaries for optimizing the results,
see
2D-LC User Guide
If this option is off, it works as a standard 2D-LC valve without dilution.
If this option is on, the user can set how often he wants to flush the sample
loop during the ASM phase.
This action activates the Active Solvent Modulation.
The ASM Factor is a read-only value and cannot be changed.
"2D-LC Capillaries Configuration Tool"
"Connecting the 2D-LC Valve, ASM (G4243A)"
2
D resolution at lowest cycle time.
on page 70.
1
D solvents in
2
D run starts.
on page 283.
140