Agilent Technologies 1290 Infinity II 2D-LC User Manual page 225

8 Run the System
Run the Experiment
Table 32
Parameter
Column
Column temperature
Stop time
Mobile Phase A
Mobile Phase B
Flow Rate
Stop time
Post time
Mobile Phase
Gradient:
Injection Volume
Injection Needle Wash In Flush Port, 10 s, acetonitrile/water (50/50)
Stop time
Diode-array Detector
Signal A
Reference Wavelength 360 nm
Reference Bandwidth
Peak width
Stop time
2D-LC User Guide
Recommended conditions in 1D (HPLC), ASM comprehensive
Value
1
D Column Compartment (MCT)
RRHD SB-C18, 2.1x 100 mm, 1.8 µm, 1200 bar (858700-902)
40 °C
As pump/No limit
1
D Pump
Water + 0.2 % formic acid
Methanol
0.1 mL/min
40 min
6 min
20 % B 0.00 min
100 % B 50 min
80 % B 7.00 min
Autosampler
2 µLfor Standard LC
0.5 µL Positive Mode for LCMS
10 µL Negative Mode for LCMS
As pump/No limit
1
D Detector (DAD)
254 nm, Bandwidth 4 nm
100 nm
20 Hz
Stop time As pump/No limit
225