Agilent Technologies 1290 Infinity II 2D-LC User Manual page 128

6 Method Parameters
Set the 2D-LC Method parameters
Comprehensive 2D-LC (LC*LC)
If you have selected comprehensive 2D-LC, the entire volume of the
injected (using the
alternating, while one loop is filled in
with the
The Modulation time reflects the duration of one injection cycle in the
that time, the solvent composition gradient will be repeated. The parameter
Modulation time is only used in the Comprehensive mode. The
time reflects the maximal duration of the gradient in
0.01 min. After that time, the Percent B value before the gradient (or the timetable
entry at time = 0.0) is restored. In the Comprehensive 2D-LC mode, the gradient
stops latest when the Modulation time is reached.
Off
Setting the mode OFF then the 2D-LC functionality is disabled. The 2D-LC
instrument is used as a standard 1D-LC instrument that allows you to carry out a
1
D run.
If you load
NOTE
the 2D-LC functionality manually.
2D-LC User Guide
2
D pump) onto the
2
D column.
1
D methods and the 2D-LC mode is still active, you must switch off
2
D column. Two identical loops are used
1
D, the volume of the other loop is separated
2
D; the smallest value is
1
D will be
2
D. After
2
D Gradient stop
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