Agilent Technologies 1290 Infinity II 2D-LC User Manual page 372

14 Theoretical Background
2D as detector
(1)
(3)
1st peak from
1st dimension
Figure 175 Principle of 2D-LC (example for LCxLC): Effluent of first column (1) is sampled (2) and
(1)
(2)
(3)
2D-LC User Guide
First dimension
Second dimension
(2)
2nd peak from
1st dimension
injected to second column (3). Peaks of second column separation are detected and
reconstructed.
First dimension separation
Sampling of the first dimension
Second dimension separation
Peak reconstruction
(software based)
(3)
(1)
3rd peak from
1st dimension
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