Agilent Technologies 1290 Infinity II 2D-LC User Manual page 208

8 Run the System
Run the Experiment
Table 19 Recommended conditions in 2D (HPLC) for multiple heart-cutting
Parameter Value
2D Gradient: Analysis 1.25 min, Equilibration 0.50 min
Analytical gradient - Shifted Gradient Shift 1D:
10 % B 0.00 min -
60 % B 1.25 min
Flush gradient not used
2
D Detector (DAD)
Diode-array 254 nm, Bandwidth 4 nm
Reference Wavelength 360 nm
Reference Bandwidth 100 nm
Peak width 80 Hz
Stop time As pump/No limit
Table 20
Parameter
Ion Source
Ion Mode
Ion polarity
Storage Mode
LCMS Stream
Acquisition Mode
Gas Temp
Gas Flow
Nebulizer
SheathGasTemp
SheathGasFlow
Scan Segment
Vcap
2D-LC User Guide
30 % B 20 min
Recommended conditions in 2D (LC-MS)
Value
Atmospheric pressure electrospray (Dual AJS ESI)
Dual AJS ESI
Both
Both
MS
Acquisition Mode MS1 Min Range (m/z) 50, Max Range (m/z) 1000, Scan
Rate (spectra/sec) 3.00
Instrument Parameters
250 °C
11 L/min
40 psig
350 °C
12 L/min
1
Scan Source Parameters
3500 V
208