Agilent Technologies 1290 Infinity II 2D-LC User Manual page 98

Solution for masshunter tof and q-tof
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4
Installation
Software Installation
Not all detectors to be configured will automatically appear in the configuration
NOTE
window. If you want to configure more detectors, you have to do it manually
using the add-on button. Please note the following notation first the module
number followed by a colon and then the serial number, for example G6135C:
US12345678.
The detector entry like module number and serial number must be correct to
avoid issues later.
For the MassHunter workflow with a High-End mass spectrometer as another
NOTE
second detector, the detector is usually not visible here. Define this transfer
volume (delay) during the file splitting in the data evaluation, see
Splitting"
4 Verify the Valves. Depending on the 2D-LC Valve you have installed, the
[OPTIONAL]
[OPTIONAL]
5 Verify the Capillaries. Select by clicking your installed capillaries. Check for
The selection of the ASM Capillary determines the ASM factor, see
NOTE
to Active Solvent Modulation (ASM)"
Therefore the ASM factor value is read only.
Generic capillaries are allowed but must be configured first in Lab Advisor before
NOTE
they show up here, see
6 To finish, leave the 2D-LC Cluster Configuration, get to the next window, click OK.
2D-LC User Guide
on page 231.
Standard 2D-LC (G4236A) or the ASM 2D-LC Valve (G4243A) will
automatically appear.
a If your system contains Multiple Heart-Cutting decks, specify which valve
head is corresponds to Deck A or B respectively will automatically appear.
b If the system contains a diverter valve, specify the role of the valve here.
You can define further diverter valve settings in the method, see
the Switch Time of the Diverter Valve"
correct loop size and correct length of the transfer capillaries. If an ASM
2D-LC Valve is used, define the ASM capillary that defines your split ratio, see
"Introduction to Active Solvent Modulation (ASM)"
To define the Sample Loop e.g. default 40 µL Sample Loop p/n 5067-5926
for MHC or 5067-5425 for SHC
To define the transfer capillary, e.g., default Capillary 0.12x170 (1.9 µL)
5500-1270 for standard valve or Capillary 0.12x170 (1.9 µL 5500-1376 for
ASM valve
To define the ASM capillary, e.g., default Capillary 0.12x170 (1.9 µL
5500-1301 for ASM valve, ASM factor 3
"2D-LC Capillaries Configuration Tool"
on page 143.
on page 35.
on page 35.
"Automated File
"Specify
"Introduction
on page 283.
98

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