Agilent Technologies 1290 Infinity II 2D-LC User Manual page 226

8 Run the System
Run the Experiment
Table 33 Recommended conditions in 2D (HPLC) for ASM comprehensive 2D-LC
Parameter Value
2D-LC Valve
MHC with 40 µl sample, Transfer Capillary, ASM Factor No
2
D Column Compartment (MCT)
Column RRHD Bonus-RP, 2.1x 50 mm, 1.8 µm, 1200 bar (857768-901)
Column temperature 40 °C
Stop time As pump/No limit
2
D Pump
Comprehensive
Figure 112 Comprehensive
Mobile Phase A Water + 0.2 % formic acid
Mobile Phase B Methanol
Flow Rate 2.5 mL/min
Stop time 40 min (will not automatically prolonged, if peaks in 2D are not work off)
Post time 6 min
2D gradient Analysis 0.2 min Equilbration 0.1 minASM Off
Analytical gradient - shifted gradient shift 1D
25 % B 0.00 min
50 % B 40 min
50 % B 0.20 min
75 % B 40 min
2
D Detector (DAD)
Diode-array 254 nm, Bandwidth 4 nm
Reference Wavelength 360 nm
Reference Bandwidth 100 nm
Peak width 80 Hz
Stop time As pump/No limit
2D-LC User Guide
25 % B 5 min
50 % B 5 min
226