14
Theoretical Background
Solvent Elution Modes
Table 46
Criterion
Peak capacity
Diversity of samples
(complex samples)
Baseline performance
(sensitivity
Pressure stress
(column lifetime!)
All modes are easily available with the Agilent 2D-LC Acquisition software.
Each mode has advantages and disadvantages. No single mode is superior in all
applications of 2D-LC.
2D-LC User Guide
Different elution modes in the
Gradient/Shifted gradient
Superior
Superior
Inferior
(baseline drift caused by solvent
gradient)
Inferior
(large changes within every 2nd
dimension gradient
2
D (pros and conts)
Isocratic/Advancing isocratic
Inferior
Inferior
Superior
Superior
(no pressure changes with
isocratic, gradually changing with
advancing isocratic)
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