Agilent Technologies 1290 Infinity II 2D-LC User Manual page 220

8 Run the System
Run the Experiment
Table 29 Recommended conditions in 2D (HPLC) for ASM MHC 2D-LC
Parameter Value
2D-LC Valve
MHC with 40 µl sample, Transfer Capillary, ASM Factor 3
2
D Column Compartment (MCT)
Column RRHD Bonus-RP, 2.1x 50 mm, 1.8 µm, 1200 bar (857768-901)
Column temperature 40 °C
Stop time As pump/No limit
2
D Pump
Heart-Cutting (time or peak based)
Figure 110 Time-based
Figure 111 Peak-based
The Cut-Time (MHC) can vary slightly depending on the configuration and the used hardware.
Mobile Phase A Water + 0.2 % formic acid
Mobile Phase B Acetonitrile
Flow Rate 1.0 mL/min
Stop time ca. 40 min (will not automatically prolonged, if peaks in 2D are not work off)
Post time 6 min
2D-LC User Guide 220