Agilent Technologies 1290 Infinity II 2D-LC User Manual page 142

6 Method Parameters
Set the 2D-LC Method parameters
Modulation
The Modulation section shows the 2D-LC Cycle/Modulation time, which is the
sum of the analysis time and the equilibration time specified in the Gradient
Phases section. The modulation time also depends on the sample loop built into
the instrument, see
values are read-only and cannot be edited.
Heart cutting
Cyle/Modulation time
Loop volume
The info of the loop filling in heart cutting is displayed in the sampling table.
NOTE
Comprehensive
Cycle/Modulation time
Loop volume
Loop filling
If the loop filling for LCxLC is smaller than 20 % or higher than 80 %, a notification
NOTE
triangle will be displayed.
2D-LC User Guide
"Recommendations for Instrument Setup"
The cycle time reflects the duration of an LC-LC injection cycle in the second
dimension. After that time, the solvent composition gradient for the next cut
will be repeated.
The Loop volume represents the configured sample loop volume.
The Modulation time reflects the duration of one LCxLC injection cycle in the
second dimension. After that time, the solvent composition gradient will be
repeated.
Loop volume represents the configured sample loop volume.
Loop filling represents the actual loop filling value.
on page 60. These
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