14
Theoretical Background
Solvent Elution Modes
Effect of shifted gradient elution mode in the
Figure 180
sg
gradient elution mode. This can lead to an improved peak detection and
improved separation.
See D. Li and O. J. Schmitz "Use of Shift Gradient in the Second Dimension to
Improve the Separation Space in Comprehensive Twodimensional Liquid
Chromatography" Anal. Bioanal. Chem. 405, 6511-6517 (2013)
2D-LC User Guide
2
D gradient mode compared to isocratic mode and its effect on resolution
as achieved in shifted gradient mode is larger than
2
D
Gradient mode
Shifted gradient mode
achieved in standard
g
379