Agilent Technologies 1290 Infinity II 2D-LC User Manual page 178

6 Method Parameters
Additional Information
Multi-Inject works similar to an injection from a large sample loop. Large
NOTE
injection volumes can negatively affect
retention by starting at low percentages of B and by applying ASM. Therefore,
Multi-Inject is not recommend for volume-based isocratic separations, e.g. SEC.
2D-LC User Guide
Figure 93 Example of High Resolution sampling (5 cuts) with 5 analytical gradients
Figure 94 Example of High Resolution sampling (5 cuts) with only one analytical gradient for
all cuts
2
D separation. Consider a good
2
D
178