Agilent Technologies 1290 Infinity II 2D-LC User Manual page 145

6 Method Parameters
Set the 2D-LC Method parameters
Modify the Solvent Composition in the
Run Time of
Use this section to modify the solvent composition in the
time of the first dimension. For each setpoint in the Analytical Gradient table that
is marked in the Shift column, you can set up a gradient shift as a nested
gradient. The gradient shift is used to align the
1
D gradient composition.
Use the table to set up the shifted
1 To set up the shift gradient, select the corresponding line in the Analytical
2 Specify the time for the change of solvent composition in the Time[min] field.
3 Specify Percent B ranges from 0 – 100 % in the B[%] field.
Different start conditions in the first row may cause step gradients and RI-effects
NOTE
(density differences of the different liquid phases may cause different DAD
detection through baseline disturbances).
The selected shift check box in the analytical gradient window can only be
NOTE
deactivated by removing the corresponding event in the Gradient Shift
window.
2D-LC User Guide
1
D
Gradient table.
The shifted gradient composition changes linearly from one setpoint to the
next. Change the solvent composition at a specified time. The time axis
relates to the stop time of the
be ignored.
Change the solvent composition at a specified time. Channel A always
delivers the remaining volume, %A = (100 - %B). The solvent composition
changes linearly from one setpoint to the next.
2
D gradient Over the
2
D gradient composition with the
2
D gradient
2
D pump, a time greater than stop time
2
D gradient over the run
2
D will
1
D Time
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